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santa cruz sc  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology santa cruz sc
    Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aqp2+antibody/AQP2+Antibody/pm41930611-90-88-88
    Average 96 stars, based on 585 article reviews
    santa cruz sc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Inhibition of Endothelin system during the postnatal nephrogenic period in the rat. Its relationship with hypertension and renal disease in adulthood
    Article Snippet: .. Blots were incubated overnight at 4°C with the AQP2 antibody (mouse monoclonal anti-rat IgG1 AQP2 [sc 515770]; Santa Cruz Biotechnology, Inc., CA, USA) diluted in blocking solution (1:200), or with α-ENaC antibody (rabbit anti-rat; diluted 1:500; Santa Cruz Biotechnology, Inc. California, USA). .. Beta-tubulin was used as loading control (rabbit anti-rat beta-tubulin; Abcam Inc., Cambridge, MA, USA).

    Article Title: The Deubiquitylase USP4 Interacts with the Water Channel AQP2 to Modulate Its Apical Membrane Accumulation and Cellular Abundance
    Article Snippet: .. The remaining lysate was transferred to a spin column containing 20 μL of Protein-A-agarose (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 1 μL of AQP2 antibody and incubated for 60 min at room temperature with end-over-end mixing. ..

    Article Title: Sickle cell disease up‐regulates vasopressin, aquaporin 2, urea transporter A1, Na‐K‐Cl cotransporter 2, and epithelial Na channels in the mouse kidney medulla despite compromising urinary concentration ability
    Article Snippet: .. Slides were then incubated with a rabbit anti‐SHP‐1 (SC‐287), NFAT5 (SC‐13035, Santa Cruz Technologies) antibody at a 1:40 dilution or the rabbit anti‐AQP2 antibody described above (1:200 dilution) overnight at 4°C. ..

    Blocking Assay:

    Article Title: Inhibition of Endothelin system during the postnatal nephrogenic period in the rat. Its relationship with hypertension and renal disease in adulthood
    Article Snippet: .. Blots were incubated overnight at 4°C with the AQP2 antibody (mouse monoclonal anti-rat IgG1 AQP2 [sc 515770]; Santa Cruz Biotechnology, Inc., CA, USA) diluted in blocking solution (1:200), or with α-ENaC antibody (rabbit anti-rat; diluted 1:500; Santa Cruz Biotechnology, Inc. California, USA). .. Beta-tubulin was used as loading control (rabbit anti-rat beta-tubulin; Abcam Inc., Cambridge, MA, USA).

    other:

    Article Title: Optical Clearing in the Kidney Reveals Potassium-Mediated Tubule Remodeling.
    Article Snippet: For the measurement of the distal convoluted tubule length, kidney-specific Kcnj10 deletion was carried out in 8 to 12-week-old mice homozygous for the floxed Kcnj10 gene (Cuevas et al., 2017) and hemizygous for the Pax8-rtTA/LC-1-Cre transgene (Traykova-Brauch et al., 2008) by providing doxycycline (2 mg/ml; Alfa Aesar, Cat#J60579) in 5% sucrose/drinking water for 3 weeks.

    Article Title: Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice
    Article Snippet: Antibodies that were used in the study are summarized in . table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Manufacturer Species Dilution (IB) Dilution (IF) AQP2 Santa Cruz Biotechnology Goat 1:5,000 1:500 TfR Abcam Rabbit 1:2,000 — GAPDH Thermo Fisher Scientific Mouse 1:10,000 — β-Actin Sigma-Aldrich Mouse 1:10,000 — p-RhoA Santa Cruz Biotechnology Mouse 1:1,000 — RhoA Santa Cruz Biotechnology Mouse 1:1,000 — p-RAC1 Santa Cruz Biotechnology Mouse 1:1,000 — RAC1 Santa Cruz Biotechnology Rabbit 1:1,000 — AQP2 p256 PhosphoSolutions Rabbit 1:3,000 — AQP2 p264 PhosphoSolutions Rabbit 1:3,000 — AQP2 p269 PhosphoSolutions Rabbit 1:3,000 — AQP2 p261 PhosphoSolutions Rabbit 1:3,000 — Open in a separate window IB, immunoblotting; IF, immunofluorescence; AQP2, aquaporin-2; TfR, transferrin receptor 1; p-RAC1, phosphorylated Ras-related C3 botulinum toxin substrate 1; p256, phosphorylated serine-256.

    Immunoprecipitation:

    Article Title: Regulation of the water channel aquaporin-2 by cullin E3 ubiquitin ligases.
    Article Snippet: Aquaporin 2 (AQP2) is a vasopressin (VP)-regulated water channel in the renal collecting duct.. Phosphorylation and ubiquitylation of AQP2 play an essential role in controlling the cellular abundance of AQP2 and its accumulation on the plasma membrane in response to VP.. Cullin-RING ubiquitin ligases (CRLs) are multisubunit E3 ligases involved in ubiquitylation and degradation of their target proteins, eight of which are expressed in the collecting duct.



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    Connexin 43 (Cx43) expression in different cellular populations of renal tissue sections from control (C) and diabetic patients (DM). Representative photomicrographs show Cx43 (Cx43, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 <t>(AQP2,</t> red), a marker for collecting duct epithelium; ( b ) synaptopodin, a marker for podocytes (synaptopodin, red) and ( c ) PDGFRB, a marker for pericytes and mesangial cells (PDGFRB, red). g—glomerulus; arrowheads indicate positive Cx43 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all).
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    Image Search Results


    Connexin 43 (Cx43) expression in different cellular populations of renal tissue sections from control (C) and diabetic patients (DM). Representative photomicrographs show Cx43 (Cx43, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) synaptopodin, a marker for podocytes (synaptopodin, red) and ( c ) PDGFRB, a marker for pericytes and mesangial cells (PDGFRB, red). g—glomerulus; arrowheads indicate positive Cx43 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all).

    Journal: International Journal of Molecular Sciences

    Article Title: Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney Disease

    doi: 10.3390/ijms27052152

    Figure Lengend Snippet: Connexin 43 (Cx43) expression in different cellular populations of renal tissue sections from control (C) and diabetic patients (DM). Representative photomicrographs show Cx43 (Cx43, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) synaptopodin, a marker for podocytes (synaptopodin, red) and ( c ) PDGFRB, a marker for pericytes and mesangial cells (PDGFRB, red). g—glomerulus; arrowheads indicate positive Cx43 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all).

    Article Snippet: , Anti-Aquaporin 2/AQP2 (E-2) , sc-515770 , Mouse , 1:50 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA.

    Techniques: Expressing, Control, Marker, Staining

    Pannexin 1 (PANX1) expression in different cellular populations of renal tissue sections from control (C) and diabetic (DM) patients. Representative photomicrographs show PANX1 (PANX1, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) nephrin (nephrin, red) a marker for podocytes. Arrowheads indicate positive PANX1 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all). g—glomerulus.

    Journal: International Journal of Molecular Sciences

    Article Title: Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney Disease

    doi: 10.3390/ijms27052152

    Figure Lengend Snippet: Pannexin 1 (PANX1) expression in different cellular populations of renal tissue sections from control (C) and diabetic (DM) patients. Representative photomicrographs show PANX1 (PANX1, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) nephrin (nephrin, red) a marker for podocytes. Arrowheads indicate positive PANX1 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all). g—glomerulus.

    Article Snippet: , Anti-Aquaporin 2/AQP2 (E-2) , sc-515770 , Mouse , 1:50 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA.

    Techniques: Expressing, Control, Marker, Staining